Regulatory

Part:BBa_K2128008:Design

Designed by: Sunil Deochand, David Sanderson   Group: iGEM16_Genspace   (2016-09-22)


H. dujardini ACTN promoter w/ 5' UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal SapI site found at 280


Design Notes

Sequence was taken by identifying the actin gene in the genome of Hypsibius dujardini. 500 bp upstream of the actin start codon was taken and subsequently ran through a promoter prediction tool which can be found at fruitfly.org.


Source

Hypsibius dujardini

References